Table 1.
Commercially available columns used for glycopeptide analysis.
| Stationary phase | UPLC/nanoLCa | Brand name | Stationary phase structure | Property of packing material | Column IDb | Reference example |
|---|---|---|---|---|---|---|
| C18 | C18 nano LC column | Acclaim PepMap C18 (Thermo Fisher) |
|
particle size: 3/5 μm pore size: 100 Å |
75/100 μm | (Li et al., 2020) |
| Nano cHiPLC C18 (Sciex) | particle size: 3/5 μm pore size: 120/250/300 Å |
75 μm | (Kozlik et al., 2017) | |||
| PGC | PGC UPLC column | Hypercarb (Thermo Fisher) |
|
particle size: 3/5/7 μm pore size: 250 Å |
1/2.1/3/4.6 mm | (Zhu et al., 2020b) |
| PGC nano LC column | 75/100 μm (discontinued) | NA | ||||
| PGC nano LC column | PGC-chip (Agilent) | particle size: 3 μm pore size: 250 Å |
43 mm chip with 40 nL trap column | (Alley et al., 2009) | ||
| HILIC | HALO nano LC column | cHiPLC HALO HILIC (Eksigent) |
|
particle size: 3 μm pore size: 120/300 Å |
75 μm | (Kozlik et al., 2017) |
| HALO HILIC (Advanced Materials Technology) | particle size: 2.7 μm pore size: 90 Å |
75 μm | (Kozlik et al., 2018) | |||
| Zwitterionic UPLC column | SeQuant ZIC-HILIC column (EMD Millipore) |
|
particle size: 3.5/5 μm pore size: 100/200 Å |
2.1/4.6 mm | (Furuki and Toyo’oka, 2017) | |
| SeQuant ZIC-cHILIC column (EMD Millipore) |
|
particle size: 3 μm pore size: 100 Å |
2.1 mm | (Furuki and Toyo’oka, 2017) | ||
| Amide UPLC column | BEH Ethylene Bridged Hybrid amide (Waters) |
|
particle size: 1.7 μm pore size: 130 Å |
2.1/3/4.6 mm | (Furuki and Toyo’oka, 2017) | |
| Accucore Amide (Thermo Fisher) |
|
particle size: 2.6 μm pore size: 150 Å |
2.1/3/4.6 mm | (Camperi et al., 2020) | ||
| Amide nano LC column | Accucore Amide (Thermo Fisher) | 75 μm | NA |
a.
NanoLC columns are marked with underline
b.
Most columns provide column with length: 5/10/15/25 cm; C18 columns provide extralong column with length 50 cm