. Author manuscript; available in PMC: 2023 Mar 1.

Published in final edited form as: Mass Spectrom Rev. 2022 Feb 4;42(2):887–917. doi: 10.1002/mas.21771

Table 3.

The strengths and weaknesses of various glycopeptide quantitation methods^a.

Glycopeptide Quantitation Method			Sensitivity	Precision	Throughput	Sample Prep Simplicity	MS Acquisition Method Simplicity	Data Simplicity
MS1 Level	Label Free	-	++	++	++++	+++++	+++++	++++
	Label- Based	Label on the Peptide	++	+++	++++	+	+++++	+++
		SILAC Labeling	++	+++	++++	+	+++++	+++
		Label on the Glycan	++	+++	++++	+	+++++	+++
		Isotope-Tagged Cleavable Linker	++	+++	++++	+	+++++	+++
MS/MS Level	Non-Targeted	TMT/iTRAQ Label-Based Approach	++	+++++	+++	+	+++++	++++
		MSE Approach	+	+	+	+++++	+++++	+
		DIA Approach	++++	++++	+++++	+++++	++++	+
	Targeted	MRM Approach	+++++	++	+++++	+++++	++	+++++
	Targeted	PRM Approach	++++	+++++	+++	+++++	+++	++

^a.

More “+” marks indicate better performance.