FIG. 2.

Biomass concentrations in continuous cultures of strains CEN-PK113-7D (wild type) and SCU10. Galactose-limited continuous cultures of strain CEN-PK113-7D (■) and SCU10 (○) were grown at different dilution rates, yielding different doubling times. At every steady state, samples were taken for 3 successive days for analyses of cell numbers (A) and dry weights (B). SEMs for dry weights and cell numbers were always <0.15 g/liter and <36 × 10⁶ cells/ml, respectively. By dividing the dry weight levels by the cell numbers at every doubling time, dry weights per cell were calculated (C). For the wild-type strain CEN-PK113-7D, also the amounts of trehalose and glycogen as shown in Fig. 3 were subtracted from the biomass per cell (□).