FIG. 3.

Carbon fluxes in continuous cultures of strain CEN-PK113-7D (wild type) and SCU10. Galactose-limited continuous cultures of strain CEN-PK113-7D (■) and SCU10 (○) were grown at different dilution rates, yielding different doubling times. At every steady state, samples were taken for 3 successive days for analyses of residual galactose, from which the consumption rate was calculated (see Materials and Methods) (A). CO₂ production was measured online (SEM < 3.6 fmol/cell/h (B). ATP production (C) was calculated from the CO₂ production rate.