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Reaction with respect to cell cycle progression after an increase in extracellular sugar concentration. From galactose-limited continuous cultures growing with average doubling times of 30 h, a sample was taken and diluted 10 times in YNB–2 mM galactose. At different time points, percentages of budded cells (A) and cell numbers (B) and trehalose and glycogen levels in strain CEN-PK113-7D (C) were determined. ■, wild-type CEN-PK113-7D; ○, mutant SCU10; ▴, trehalose; □, glycogen.
