Fig. 3.

Sensitivity of mutant strains to ABZ. Strains are presented in rank order of ABZ resistance after 3 days of growth at 20°C, during which time controls grew to L4/Adulthood. a) Growth of ben-1 mutations relative to wild type cultured in parallel (a representative wild type is shown). b) Growth on 7.5 μM ABZ was normalized to the average of the same strain grown in parallel in the absence of drug shown in (a). sb151 and sb163 showed the least resistance. c) Growth of the wild type and sb156, which does not have a lesion in the ben-1 coding region, relative to the wild type. d) Assays run in parallel to (c) at the indicated levels of ABZ. sb156 shows partial resistance at the lower dose. Two-tailed Mann–Whitney Rank-Sum test were used to calculate P-values. Mean and standard deviations are indicated. Supplementary Fig. 6 contains raw data in microns.