Table 7.
Characteristics of gene families involved in essential oil biosynthesis in M. longifolia.
| Gene annotation | Mentha longifolia chromosome | Location identifier | NCBI accession code | Number of introns | DNA strand | Expression in glandular trichomes [TPM]a | Homology to canonical genes (amino acid level)b |
|---|---|---|---|---|---|---|---|
| Terpene synthases (E.C. 4.2.3.- or EC 5.5.1.-) | |||||||
| (−)-Limonene synthase | |||||||
| LS-1 | 5 | Mlong585_17628 | MT664991 | 6 | (−) | 5,246 | 98% identity to Q40322 |
| LS-2 | 5 | Mlong585_17636 | MT664992 | 6 | (−) | As above | 98% identity to Q40322 |
| Oxidoreductases that incorporate 1 atom of oxygen (EC 1.14.13.- or EC 1.14.14.-) | |||||||
| (−)-Limonene 3-hydroxylase | |||||||
| L3H-1 | 5 | Mlong585_17637 | MT664993 | 1 | (−) | 2,281 | 99% identity to Q9XHE7 |
| L3H-2 | 5 | Mlong585_17629 | MT664994 | 1 | (−) | As above | 99% identity to Q9XHE7 |
| L3H-3 | 9 | Mlong585_30867 | MT664995 | 1 | (+) | 2,475 | 97% identity to Q9XHE7 |
| L3H-4 | 9 | Mlong585_30878 | MT664996 | 1 | (+) | As above | Pseudogene |
| (+)-Menthofuran synthase | |||||||
| MFS-L1 | 2 | Mlong585_05231 | n.a. | 2 | (+) | 1,180 | 90% identity to Q947B7 |
| Oxidoreductases acting on CH-OH groups of donors (EC 1.1.1.-) | |||||||
| (−)-Isopiperitenol/(−)-carveol dehydrogenase | |||||||
| ISPD-1 | 2 | Mlong585_04858 | MT664997 | 0 | (−) | 137 | 98% identity to Q5C9I9 |
| ISPD-2 | 2 | Mlong585_04874 | MT664998 | 0 | (+) | As above | 98% identity to Q5C9I9 |
| ISPD-L1 | 2 | Mlong585_04859 | MT664999 | 0 | (−) | 127 | 80% identity to Q5C9I9 |
| ISPD-L2 | 2 | Mlong585_04873 | MT665000 | 0 | (+) | As above | 80% identity to Q5C9I9 |
| (−)-Menthone:(−)-menthol reductase | |||||||
| MMR-L1 | 11 | Mlong585_36607 | n.a. | 6 | (−) | 107 | 87% identity to Q9XHE7 |
| (−)-Menthone:(+)-neomenthol reductase-like | |||||||
| MNR-L1 | 11 | Mlong585_36608 | n.a. | 0 | (−) | n.a. | Pseudogene |
| Oxidoreductases acting on CH-CH groups of donors (EC 1.3.1.-) | |||||||
| (−)-Isopiperitenone reductase | |||||||
| ISPR-1 | 11 | Mlong585_36604 | MT665001 | 4 | (−) | 20 | 94% identity to Q6WAU1 |
| ISPR-2 | 11 | Mlong585_36605 | MT665002 | 4 | (+) | As above | 99% identity to Q6WAU1 |
| (+)-Pulegone reductase | |||||||
| PulR-1 | 3 | Mlong585_09229 | MT665003 | 4 | (−) | 3,116 | 95% identity to Q6WAU0 |
| PulR-2 | 3 | Mlong585_09276 | MT665004 | 4 | (−) | As above | 95% identity to Q6WAU0 |
| PulR-3 | 3 | Mlong585_09230 | MT665005 | 0 | (+) | n.a. | Pseudogene |
| PulR-L1 | 2 | Mlong585_06346 | MT665006 | 4 | (−) | n.a. | 83% identity to Q6WAU0 |