FIG. 2.

Purification of SLN1-R1 and SSK1-R2 domains using the IMPACT fusion protein system. Protein expression and purification of SLN1-R1 (A) and SSK1-R2 (B) were analyzed by SDS-PAGE. Lanes 1, whole-cell lysates from uninduced cells; lanes 2, whole-cell lysates of induced cells, grown for 8 h at room temperature; lanes 3, soluble protein fraction after cell lysis; lanes 4, pooled fractions from chitin column following overnight thiol-induced cleavage; lanes 5, pooled fractions after gel filtration. Proteins were denatured in SDS-PAGE sample buffer in the absence of reducing agents, separated on SDS–15% polyacrylamide gels, and stained with Coomassie blue. Sideways carets (<) indicate induced expression of full-length fusion protein. Sizes of molecular markers are given on the left.