Fig. 2.

FMRP targets encoding large proteins are downregulated in the mouse cortex. a, a′) Profiles and quantification of capillary electrophoresis western experiments showing a partial reduction in the levels of FMRP targets Auts2 and Med13L and complete loss of FMRP in the Fmr1 KO mouse cortex as compared to wild-type controls. Insets show standard curves of western signal as a function of total protein concentration. b) Total protein levels of all biotin-labeled proteins in mouse cortex extracts. b′) A zoom-in of (b) showing large proteins >300 kDa. b″) Quantification of (b) showing a decrease in the levels of large proteins but not total proteins in Fmr1 KO mouse cortex samples. c) Quantification of capillary electrophoresis western experiments of indicated proteins performed on mouse cortex extracts from wild-type or Fmr1 KO animals. *P < 0.05, ^#P < 0.1.