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Calpain-1 (CAPN1) protein expression was determined on (A) day 2 and (B) 10 post-implanting by SDS-PAGE and Immunoblotting as described in the Materials and Methods. Steers were stratified by weight and by breed; Angus (AN) or Santa Gertrudis (SG) and assigned to one of two treatments: (1) Control, no implant (CON), or (2) implanted with 120 mg trenbolone acetate and 24 mg estradiol (IMP). Data represent CAPN1 abundance and are presented as LS mean ± SEM.
