Table 4.
Abundance of receptor mRNA associated with skeletal muscle growth from longissimus thoracis skeletal muscle of feedlot steers of different cattle breed types following implanting
| Steers (n) | Implant and breed treatments1 | SEM |
P-values2 | |||||
|---|---|---|---|---|---|---|---|---|
| Gene3 | AN-IMP | SG-IMP | AN-CON | SG-CON | Breed | Trt | B × T | |
| 5 | 5 | 5 | 5 | |||||
| OXTR | ||||||||
| Day 2 | 56.58 | 19.56 | 69.90 | 99.98 | 50.91 | 0.94 | 0.24 | 0.37 |
| Day 10 | 0x | 23.21xy | 0x | 88.58y | 32.01 | 0.03 | 0.15 | 0.31 |
| IGF-1R | ||||||||
| Day 2 | 59.65 | 23.46 | 25.83 | 18.00 | 14.00 | 0.15 | 0.19 | 0.35 |
| Day 10 | 21.75 | 23.01 | 31.87 | 37.65 | 10.08 | 0.72 | 0.19 | 0.80 |
| VDR | ||||||||
| Day 2 | 77.05 | 25.26 | 62.18 | 104.46 | 48.13 | 0.92 | 0.47 | 0.29 |
| Day 10 | 6.93a | 22.91a | 4.66a | 114.93b | 22.54 | 0.01 | 0.04 | 0.03 |
Implant treatments administered on day 0 include the following: no implant +Angus (AN-CON), no implant + Santa Gertrudis (SG-CON), Revalor-S + Angus (AN-IMP; 120 mg trenbolone acetate + 24 mg estradiol) and Revalor-S + Santa Gertrudis (SG-IMP; 120 mg trenbolone acetate + 24 mg estradiol).
P-values indicate the effect of Breed, Treatment (TRT), or B × T (Breed × Treatment). Skeletal muscle biopsies were collected on day 2 and 10 post-implanting, and RNA isolation and quantitative PCR were performed as described in the Materials and Methods.
OXTR, oxytocin receptor; IGF-1R, insulin-ike growth factor 1 receptor; VDR, vitamin D receptor.
Different letters indicate a difference (P < 0.05) between time points.
Different letters indicate a tendency between time points (0.05 < P ≤ 0.10). All data are presented as the least square means ± SEM.
Italics indicate P-values for the different effects (breed, trt and trtxbreed).