Table 1.

Experiment 1 setup

Group	Experimental group	Vaccine	α-GalCer (μg/kg)	Challenge virusc	N
1	Mock – CO99	Vehiclea	Vehicleb	H3N2 CO99	3
2	TX98ΔNS1 + 0μg αGC – CO99	TX98ΔNS1	Vehicle	H3N2 CO99	2
3	TX98ΔNS1 + 10μg αGC – CO99	TX98ΔNS1	10	H3N2 CO99	3
4	TX98ΔNS1 + 50μg αGC – CO99	TX98ΔNS1	50	H3N2 CO99	3
5	TX98ΔNS1 + 100μg αGC – CO99	TX98ΔNS1	100	H3N2 CO99	3

^aVirus-free Dulbecco's Modified Eagle's Medium (DMEM)

^b50 μL/kg of dimethyl sulfoxide (DMSO) (the volume used to dissolve the 100 μg/kg dose of α-GalCer used in group 5)

^c1 x 10⁶ TCID₅₀ H3N2 A/Swine/Colorado/23619/1999 administered intratracheally (i.t.) in 2 mL of DMEM