Table 2.

Experiment 2 setup

Group	Experimental group	Vaccine	α-GalCer (μg/kg)	Challenge virusc	N
1	Mock – Mock	Vehiclea	Vehicleb	-	2
2	Mock – TX98	Vehicle	Vehicle	H3N2 TX98	6
3	Mock – CA04	Vehicle	Vehicle	H1N1 CA04	6
4	TX98ΔNS1 – TX98	TX98ΔNS1	Vehicle	H3N2 TX98	6
5	TX98ΔNS1 – CA04	TX98ΔNS1	Vehicle	H1N1 CA04	6
6	TX98ΔNS1 αGC – CA04	TX98ΔNS1	50	H1N1 CA04	6

^aVirus-free DMEM

^b25 μL/kg of DMSO (the volume used to dissolve the 50 μg/kg dose of α-GalCer used in group 6)

^c1 x 10⁶ TCID₅₀ H3N2 A/Swine/Texas/4199-2/1998 or H1N1 A/California/04/2009 administered i.t. in 2 mL of DMEM