Figure 2.
Immunoblot analyses of proteins regulating glutaminolysis and lipogenesis in nonadherent MIAPaCa-2 (mutant KRAS) and BxPC3 (wild-type KRAS) cancer cells treated with CPT. A total of 5×105 cells were seeded into poly-HEMA-coated 60 mm dishes and treated with CPT at 10 and 20 µM for 48 h. GAPDH was used as the internal control. KRAS, Kirsten rat sarcoma viral oncogene homolog; CPT, cryptotanshinone; GOT, glutamic-oxaloacetic transaminase; GLUD, glutamate transport system permease protein; GLS, glutaminase; IDH, isocitrate dehydrogenase; FASN, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; ACLY, ATP-citrate lyase; p-, phosphorylated.