Figure 4.

Immunoblot analyses of proteins regulating glutaminolysis and lipogenesis in MIAPaCa-2 cells treated with control (siC) and KRAS siRNA (siKRAS). (A) A total of 1×10⁵ cells were treated with siRNA for 24 h, followed by nonadherent culture (48 h). GAPDH was used as the internal control. (B) Quantitative analysis of protein expression levels of K-Ras, FASN and ACC1 after siKRAS treatment in MIAPaCa-2 cells. The bars indicate the relative expression value normalized to those of GAPDH and are presented as mean ± standard deviation of three independent assays. ^*P<0.05 compared with siC using Welch's t-test. KRAS, Kirsten rat sarcoma viral oncogene homolog; si, short interfering; FASN, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; ACLY, ATP-citrate lyase; GOT, glutamic-oxaloacetic transaminase; IDH, isocitrate dehydrogenase; GLUD, glutamate transport system permease protein; GLS, glutaminase; p-, phosphorylated.