Extended Data Fig. 4. Inactivation of intratumoral CTL and robust tumor growth require IFNAR1-dependent function of PARP11 in the TME.

A. Flow cytometry analysis of levels of IFNAR1 on the surface of CD45⁺CD3⁺CD8⁺ T cells in tumor tissues from WT, Parp11^−/−, and Parp11^−/−Ifnar1^−/− mice 13 days after inoculation of s.c. B16F10 tumors (0.5×10⁵/ mouse). Statistical analysis was performed using ordinary one-way ANOVA with Tukey`s multiple comparisons test. n = 5 mice.

B. Growth of s.c. LLC tumors (1×10⁶/ mouse) inoculated into WT or Parp11^−/− mice. Statistical analysis was performed using two-way ANOVA with Tukey`s multiple comparisons test. ****P < 0.0001. WT, n = 6 mice; Parp11^−/−, n= = 5 mice.

C. Growth of s.c. B16F10 tumor cells (0.5×10⁵/ mouse) inoculated into WT, Parp11^−/−, or Parp11^−/−Ifnar1^−/− mice. Statistical analysis was performed using two-way ANOVA with Tukey`s multiple comparisons test. *P =0.0387, ****P < 0.0001. n = 5 mice.

D. Quantification of B16F10 tumor weights in mice of indicated genotypes at day 13 after inoculation. Statistical analysis was performed using ordinary one-way ANOVA with Tukey`s multiple comparisons test. ****P < 0.0001. n = 5 mice.

E. Flow cytometry analysis of numbers of CD8⁺ T cells in B16F10 tumors growing in indicated mice. Statistical analysis was performed using ordinary one-way ANOVA with Tukey`s multiple comparisons test. ***P = 0.0007. n = 5 mice.

F. Flow cytometry analysis of IFN-γ⁺ cells gated on CD45⁺CD3⁺CD8⁺ T cells in B16F10 tumors growing in indicated mice. Statistical analysis was performed using ordinary one-way ANOVA with Tukey`s multiple comparisons test. n = 5 mice.

G. Flow cytometry analysis of TNF-α⁺ cells gated on CD45⁺CD3⁺CD8⁺ T cells in B16F10 tumors growing in indicated mice. Statistical analysis was performed using ordinary one-way ANOVA with Tukey`s multiple comparisons test. n = 5 mice.

H. Flow cytometry analysis of CD69⁺ cells gated on CD45⁺CD3⁺CD8⁺ T cells in B16F10 tumors growing in indicated mice. Statistical analysis was performed using ordinary one-way ANOVA with Tukey`s multiple comparisons test. n = 5 mice.