|
Antibodies
|
| Goat anti-rabbit AlexaFluor-488 |
ThermoFisher |
A11008; RRID:AB_143165 |
| Rabbit polyclonal anti-TCOF1 |
ThermoFisher |
PA558309; RRID:AB_2648324 |
| Rabbit polyclonal anti-SALL1 |
Invitrogen |
PA562057; RRID:AB_2646913 |
| Rabbit polyclonal anti-BARD1 |
abcam |
ab226854 |
|
Biological samples
|
| Human adult large intestine adjacent normal tissue |
BioIVT |
HMLGINTAD J2-PPG7 |
| Human adult breast adjacent normal tissue |
BioIVT |
HMNBREASTN ADJ2 |
| Human adult kidney normal |
BioIVT |
HMKIDNEYNO RC2 |
| Chemicals, peptides, and recombinant proteins |
| Poly-L-ornithine |
Sigma |
P4957-50ML |
| Doxycycline |
Sigma |
D9891-5G |
| Hoechst 33258 |
Life Technologies |
H3569 |
| Vectashield |
Vector Laboratories |
101098-042 |
| Laminin (Mouse) |
ThermoFisher |
CB40232-1MG |
| Critical commercial assays |
| Lipofectamine 3000 Transfection Agent |
ThermoFisher |
L3000001 |
| Experimental models: Cell lines |
| V6.5 murine embryonic stem cells |
Laboratory of Rudolph Jaenisch |
N/A |
| Human: HCT116 |
ATCC |
CCL-247 |
| Human: MCF7 |
ATCC |
HTB-22 |
| Human: HEK293T |
ATCC |
CRL-3216 |
| Recombinant DNA |
| pbfh_GFP |
(Henninger et al., 2021) |
Modified version of pJH135_pb_MCPx2_mCherry_rTTA |
| pbfh_GFP_MECP2 |
This study |
N/A |
| pbfh_GFP_MECP2_R186* |
This study |
N/A |
| pbfh_GFP_BARD1 |
This study |
N/A |
| pbfh_GFP_BARD1_R406* |
This study |
N/A |
| pbfh_GFP_BCL11A |
This study |
N/A |
| pbfh_GFP_BCL11A_Q177* |
This study |
N/A |
| pbfh_GFP_BCOR |
This study |
N/A |
| pbfh_GFP_BCOR_Y657* |
This study |
N/A |
| pbfh_GFP_BRD3 |
This study |
N/A |
| pbfh_GFP_BRD3_F334S |
This study |
N/A |
| pbfh_GFP_HP1A |
This study |
N/A |
| pbfh_GFP_HP1A_V21I |
This study |
N/A |
| pbfh_GFP_HP1A_W142C |
This study |
N/A |
| pbfh_GFP_DAXX |
This study |
N/A |
| pbfh_GFP_DAXX_R318* |
This study |
N/A |
| pbfh_GFP_EDC4 |
This study |
N/A |
| pbfh_GFP_EDC4_I371V |
This study |
N/A |
| pbfh_GFP_ESRP1 |
This study |
N/A |
| pbfh_GFP_ESPR1_L259V |
This study |
N/A |
| pbfh_GFP_NONO |
This study |
N/A |
| pbfh_GFP_NONO_446fs |
This study |
N/A |
| pbfh_GFP_RBM10 |
This study |
N/A |
| pbfh_RBM10_V354M |
This study |
N/A |
| pbfh_GFP_SALL1 |
This study |
N/A |
| pbfh_GFP_SALL1_S372* |
This study |
N/A |
| pbfh_GFP_SRSF2 |
This study |
N/A |
| pbfh_GFP_SRSF2_S54F |
This study |
N/A |
| pbfh_GFP_SRSF2_P95H |
This study |
N/A |
| pbfh_GFP_TCOF |
This study |
N/A |
| pbfh_GFP_TCOF_Q55* |
This study |
N/A |
| pbfh_GFP_ASXL1 |
This study |
N/A |
| pbfh_GFP_BCL6 |
This study |
N/A |
| pbfh_GFP_BRCA1 |
This study |
N/A |
| pbfh_GFP_DVL2 |
This study |
N/A |
| pbfh_GFP_DYR1A |
This study |
N/A |
| pbfh_GFP_ENC1 |
This study |
N/A |
| pbfh_GFP_G3BP1 |
This study |
N/A |
| pbfh_GFP_HMGA2 |
This study |
N/A |
| pbfh_GFP_NIPBL |
This study |
N/A |
| pbfh_GFP_NKX21 |
This study |
N/A |
| pbfh_GFP_SNCAP |
This study |
N/A |
| pbfh_GFP_TERT |
This study |
N/A |
| Software and algorithms |
| Fiji image processing package |
(Schindelin et al., 2012) |
https://fiji.sc/
|
| Prism |
GraphPad |
https://www.graphpad.com/scientific-software/prism/
|
| Code generated by the study |
This study |
https://github.com/bananisf/
|
| Python v3.6.9 |
www.python.org
|
N/A |
| CellPose v0.7.2 |
(Stringer et al., 2021) |
http://www.cellpose.org/
|
| MATLAB R2019b |
Mathworks |
N/A |
| metapredict |
(Emenecker et al., 2021) |
N/A |
| PLAAC |
(Lancaster et al., 2014) |
N/A |
| PSP |
(Vernon et al., 2018) |
N/A |
| Ensembl VEP v102 |
(Yates et al., 2019) |
N/A |
| Other |
| 35 mm glass-bottom imaging dishes |
Mattek Corporation |
P35G-1.5-20-C |
