Fig. 7.

Effects of ADAM17 or ACE2 deficiency on autophagy-related proteins. a, b Representative Western blot images and quantitative analyses of protein expression of Beclin1, Atg3, Atg5, Atg7, and Atg12 in four groups of differentiated H9c2 cells treated with vehicle, GP, GP + NC-siRNA and GP + ADAM17-siRNA, respectively. c, d Representative Western blot images and analysis of phosphorylated mTOR, mTOR, phosphorylated p70S6K, p70S6K, phosphorylated 4EBP1 and 4EBP1 protein expression in differentiated H9c2 cells treated as above. Mean values were derived from 5 independent experiments. e–g Representative Western blot images and quantitative analysis of phosphorylated AMPK, AMPK, and TFEB protein expression in differentiated H9c2 cells treated with vehicle, GP, GP + NC-siRNA and GP + ACE2-siRNA, respectively. h–k Representative Western blot images and analysis of phosphorylated mTOR, mTOR, p62 and Beclin1 protein expression in differentiated H9c2 cells treated as above. Mean values were obtained from five independent experiments. Data were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the vehicle group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. the GP + NC-siRNA group