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. 2022 Jul 18;13:926622. doi: 10.3389/fendo.2022.926622

Figure 2.

Figure 2

Effects of C91 on Wnt/β-catenin signaling pathway in ST2 cells. (A) ST2 cells were treated with C91 for 12 and 24 hours, and the samples were collected for immunofluorescence staining analysis of β-catenin. The red arrows indicate the protein of β-catenin into the nucleus. Scale bar = 50 μm. (B) Western blot detection of protein β-catenin expression. (C) TopFlash is a reporter gene plasmid used to detect the level of β-catenin-mediated TCF/LEF transcriptional activity in Wnt signaling pathway, and FopFlash plasmid is its negative control. ST2 cells were transfected with TopFlash and FopFlash plasmids and then treated with C91, L-ctrl and Wnt3a conditioned medium for 3 days. Samples were collected and assayed for luciferase activity. (D) qRT-PCR detection of Wnt target gene expression. Results are expressed as mean ± SD (n = 3 per group). * indicates P < 0.05, compared with the DMSO group. # indicates P < 0.05, compared with the C91 group.