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. 2022 Jul 18;13:926622. doi: 10.3389/fendo.2022.926622

Figure 3.

Figure 3

Effects of inhibition of Wnt/β-catenin signaling pathway on ST2 cell differentiation. Triptonide (Tript) is a Wnt signaling inhibitor. ST2 cells were treated with C91 and Tript (10nM) for 3 days, and the samples were collected for AP staining analysis (A) and AP biochemical quantitative analysis (B). Positive cells, scale bar = 100 μm. (C, F) qRT-PCR detection of osteogenic target genes and Wnt target gene mRNA expression levels. (D) ST2 cells were treated with Tript (10 nM) and C91 for 14 days, and the osteogenic induction medium was changed every two days in between. Alizarin red S staining was used to detect mineralized nodules. The lower image is a 10× enlargement of the upper image. Red arrows are mineralized nodules, scale bar = 50 μm. (E) is the quantitative analysis result of panel (D) Results are expressed as mean ± SD (n = 3 per group). * indicates P < 0.05, compared with the DMSO group. # indicates P<0.05, compared with the C91 group.