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. 2022 Jul 18;13:926622. doi: 10.3389/fendo.2022.926622

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Copyright © 2022 Wang, Khan, Wang, Wang, Liu, Chen and Tu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Study the relationship between autophagy and Wnt/β-catenin signaling pathway. ST2 cells were treated with Wnt signaling inhibitors Triptonide(Tript) (10nM, 20nM, 40nM, 80nM) and C91 (5μM) for 3 days, and the samples were collected for AP staining analysis (A) and AP biochemical quantitative analysis (B). (C, D) qRT-PCR detection of osteogenic target gene and Wnt target gene mRNA expression. Tript at a concentration of 80nM inhibited the osteogenic differentiation of ST2 the best. Rapamycin (Rapa) is an autophagy activator. ST2 cells were treated with C91, Tript (80nM) and Rapamycin (200nM) for 3 days, and the samples were collected for AP staining analysis (E) and AP biochemical quantitative analysis (F). (G) The expression of Alpl mRNA, an early osteogenic factor, was detected by qRT-PCR. Results are expressed as mean ± SD (n = 3 per group). * indicates P<0.05, compared with DMSO group.