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. 2022 Jul 18;13:926622. doi: 10.3389/fendo.2022.926622

Figure 7.

Figure 7

Effects of C91 on ST2 cell growth and proliferation in 3D modules. (A) Schematic diagram of a PCI3D system design (left) and its printing module image (right). The C91-loaded hydrogel bundles, the ST2-loaded hydrogel bundles, and the PCL bundles were printed with each other to form one layer, and 4 layers were continuously printed to form a 3D module. (B) Live/dead cell staining was used to detect the survival and proliferation of cells 1, 4, and 7 days after in vitro culture. Live cells show calcein-am positive (green) and dead cells show EthD-1 negativity (red). Scale bar = 100 μm. (C) Live/dead cell ratios were analyzed using confocal microscopy imaging. (D) The effect of C91 in the CCK8 detection module on the proliferation activity of ST2 cells. Results are expressed as relative OD450 values, calculated by normalizing OD450 readings to the mean value of the control group. (E) The daily sustained release of C91 in the 3D module was detected using a drug release assay. * indicates P < 0.05, compared with the DMSO group.