TABLE 2.
Occurrence of hsp70 (dnaK) among archaeaa
| Organism | PCR amplification,a Southern blottingb (reference)c | Genome sequencing (reference)c |
|---|---|---|
| D. mobilis | − (this report)a,b | ND |
| S. solfataricus | − (this report)a,b | ND |
| Sulfolobus sp. | − (33)b | ND |
| T. tenax | − (this report)a,b | ND |
| M. kandleri | − (this report)a,b | ND |
| P. woesei | − (this report)a,b | ND |
| P. horikoshi | ND | −d |
| M. fervidus | − (33b; this reporta,b) | ND |
| M. thermoautotrophicum ΔH | ND | + (46) |
| M. vannielii | − (this report)a,b | ND |
| M. voltae | − (28)b | ND |
| M. jannaschii | − (33)b | − (9) |
| A. fulgidus | ND | − (32) |
| T. acidophilum | + (22)a,e | ND |
| M. mazei S-6 | + (37)b,e | ND |
| M. thermophila TM-1 | + (33)b,e | ND |
| M. hungatei | − (33)b | ND |
| H. halobium | + (this report)a,b | ND |
| H. cutirubrum | + (22)a,e | ND |
| H. marismortui | + (20)a,e | ND |
PCR amplification using the two degenerate primers for the sequences QATKDAG and NPDEAVA (see Materials and Methods).
Southern blotting with M. mazei dnaK as the probe.
ND, not done; + and −, presence and absence, respectively, of dnaK-related sequences.
Unpublished sequence; retrievable at http://www.bio.nite.go.jp/.
Organism whose dnaK has been cloned and sequenced.