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. 2022 Jul 18;9:920013. doi: 10.3389/fcvm.2022.920013

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Copyright © 2022 Maselli, Matos, Johnson, Martella, Caprettini, Chiappini, Camelliti and Campagnolo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Epicardial slices decolouration. Immunohistochemical staining and confocal analysis 3D reconstruction of decolorized epicardial slices obtained from piglet hearts, showing (a) low magnification image of the epicardial layer stained with MSLN (green) and (b) high magnification image showing the epicardial monolayer stained with MSLN (green) and WT1 (red) from the bottom. Low magnification (c) and detail view (d) of the vasculature present into the epicardial slice a stained with smooth muscle marker α-SMA (red) and the endothelial marker CD31 (green). Scale bars, 50 μm.