Table 1.
Cardioplegia | Normal Tyrode's solution | Normal Tyrode's solution for recording | ||||||
---|---|---|---|---|---|---|---|---|
Composition | Manufacturer | Concentration (mM) | Composition | Manufacturer | Concentration (mM) | Composition | Manufacturer | Concentration (mM) |
NaCl | VWR Chemicals | 110 | NaCl | VWR Chemicals | 140 | NaCl | VWR Chemicals | 140 |
CaCl2 | Sigma-Aldrich | 1.2 | CaCl2 | Sigma-Aldrich | 1.8 | CaCl2 | Sigma-Aldrich | 1.8 |
KCl | Sigma-Aldrich | 16 | KCl | Sigma-Aldrich | 6 | KCl | Sigma-Aldrich | 4.5 |
MgCl2 | VWR Chemicals | 16 | MgCl2 | VWR Chemicals | 1 | MgCl2 | VWR Chemicals | 1 |
Glucose | Sigma-Aldrich | 10 | Glucose | Sigma-Aldrich | 10 | |||
HEPES | VWR Chemicals | 10 | HEPES | VWR Chemicals | 10 | |||
BDM | Sigma-Aldrich | 10 | ||||||
*add NaHCO3 (10mM) to pH 7.4 | *add NaOH (10M) to pH 7.4 | *add NaOH (10M) to pH 7.4 |
*The component needs to be added to adjust the pH of the solution.