TABLE 2.
GS specific activities in vitro
| Strain | Genotype | GS sp acta (nmol · mg−1 · h−1) | % Glucanase-sensitive productb |
|---|---|---|---|
| H99 | Wild type | 11.4 ± 0.7 | 69 |
| 42-2-13 | A type | 5.6 ± 0.6 | 83 |
| 42-1-28 | A type | 9.6 ± 5 | 75 |
| 42-1-43 | Indeterminate | 5.1 ± 0.4 | 78 |
| 42-1-48 | Indeterminate | 6.8 ± 0.6 | 80 |
| 42-6-60 | Indeterminate | 7.0 ± 0.3 | 77 |
a
Reactions for determination of GS specific activity contained 15 mM UDPG at 1,000 dpm/nmol. Each value is the average ± standard deviation for values from three assays.
b
Glucanase susceptibility of products was measured using a final concentration of 1.5 mM UDPG at 20,000 dpm/nmol.