FIGURE 3.

Effects of bacterial exposure on monocyte-derived macrophage (MDM) apoptosis. MDMs from six COPD patients were exposed to Haemophilus influenzae (HI), Moraxella catarrhalis (MC) or Streptococcus pneumoniae (SP) at a multiplicity of infection of 5:1, Triton-X 0.1% (positive death control) or left unexposed (control) for 24 h. Cells were stained for nuclear stain DAPI and the apoptosis marker TUNEL or left unstained (negative). The percentage of positive stained cells was determined with a minimum of 50 cells per condition counted across multiple fields of view. a) Data represent mean+sem percentage cells positive for TUNEL, and b) representative overlay images of TUNEL (green) and DAPI (blue) shown. **: significantly above unexposed control (p<0.01).