Table 1.
Genetically modified live attenuated vaccines against brucellosis.
| Gene deleted | Function | Host | Vaccination (dose, route) | Challenge (Brucella species) | Challenge (weeks or days p.v.) | Protection | References |
|---|---|---|---|---|---|---|---|
| cydC, cydD, purD | ATP-binding cassette-type transporter Phosphoribosylamine–glycine ligase |
BALB/c mice | 2.4–3.1 × 108 CFU, i.p. | B. abortus 2308 | 7w 21w 7w 21w |
2.37-log 2.64- log 1.48-log 2.72- log |
(19) |
| Hfq | Regular expression of some target genes, affects mRNA stability | BALB/c mice | 1 × 106 CFU, i.p. | B. melitensis 16M | 2w 4w |
1.64 -log 2.06- log |
(20) |
| bp26 | Periplasmic or cytoplasmic protein | BALB/c mice | 1 × 106 CFU, i.p. | B. melitensis 16M | 5w | 2.89-log | (21) |
| omp31 | Outer membrane protein | BALB/c mice | 5 × 105 CFU, i.p. | B. melitensis Bm133 | 3w 6w 9w |
≈4.1- log ≈3.9- log ≈2.3- log |
(22) |
| VjbR | HTH-type-quorum-sensing-dependent transcriptional regulator | BALB/c mice | 1 × 106 CFU, i.p. | B. melitensis 16M | 2w 4w |
1.70-log 3.05-log |
(23) |
| C57BL/6 mice | 107 CFU, i.p. | B. canis RM6/66 | 1 w | 3.092- log | (24) | ||
| MucR | Transcriptional regulatory protein | BALB/c mice | 105 and 106 CFU, i.p. | B. melitensis 16M | 20w | 4.14–4.75-log | (25) |
| ZnuA | Zn2+ transport system | BALB/c mice | 3 × 1011 cells, oral | B. melitensis 16M | 4w | 3- log | (26) |
| ManB | Phosphomannomutase (LPS synthesis) | BALB/c mice | 1 × 106 CFU, i.p. | B. melitensis 16M | 2w 4w |
1.74- log 1.87- log |
(21) |
| Pgm | Phosphoglucomutase (LPS synthesis) | BALB/c mice | 1 × 106 CFU, i.p. | B. melitensis 16M | 2w 4w |
3.43-log 2.83-log |
(27) |
ip, Intraperitoneal; CFU, colony-forming unit; W, week; log, logarithm.