. 1999 Jan;181(2):462–468. doi: 10.1128/jb.181.2.462-468.1999

TABLE 2.

Biological functions of BglF individual domains or combinations of domains in vivo

Plasmid(s)	Plasmid-encoded BglF derivative	Complementation^a of bglF mutant strain PPA501 (expressing IIA^g^l^c and EIIⁿ^a^g)	β-Galactosidase activity (U)^b
Plasmid(s)	Plasmid-encoded BglF derivative		Without salicin	With salicin
pMN5	Wild-type BglF	+	5	153
pACQ-F3	IIB^b^g^l	−	3	5
pT7CQ-F4	IIC^b^g^l	−	175	178
pT7CQ-F5	IIA^b^g^l	−	225	255
pACQ-F6	IICA^b^g^l	−	724	637
pACQ-F8	IIBC^b^g^l	+	10	415
pACQ-F6/ pT7CQ-F3	IICA^b^g^l + IIB^b^g^l	−	5	5
pT7CQ-F4/ pACQ-F3	IIC^b^g^l + IIB^b^g^l	−	4	4
pBR322		−	447	456

Complementation was indicated by two alternative methods: +, growth on minimal arbutin plates and red colonies on MacConkey arbutin plates; −, no growth on minimal arbutin plates and white colonies on MacConkey arbutin plates.

Determined with strain PPA501, which carries a bgl-lacZ transcriptional fusion and a defective bglF gene and which constitutively expresses IIA^glc and EII^nag, encoded by genes crr and nagE, respectively. The values represent the averages of four independent measurements. Salicin at 7 mM was added to the growth medium when indicated.