Table 1.
Primers and probes used in this study.
| Primers | Sequence (5’-3’) | Length (bp) | Gene Name | |
|---|---|---|---|---|
| 1 | SF | AATGTTCGTGTTCTTGGTCTTGC | 3800 | S |
| pUC/M13R | AGCGGATAACAATTTCACACAGG | |||
| 2 | M1F | ATGAGCCTGTTGACCGAGGT | 750 | M1 |
| M1R | CTACTTGAAACGCTGCATC | |||
| 3 | mSF | AATGTTCGTGTTCTTGGTCTTGC | 3800 | mS |
| pUC/M13R | AGCGGATAACAATTTCACACAGG | |||
| 4 | M1F | ATGAGCCTGTTGACCGAGGT | 1500 | M1 |
| pUC/M13F | CCCAGTCACGACGTTGTAAAACG | |||
| 5 | SARS-CoV-2 NF | GGGGAACTTCTCCTGCTAGAAT | 99 | N |
| SARS-CoV-2 NR | CAGACATTTTGCTCTCAAGCTG | |||
| SARS-CoV-2 NP | 5’-FAM-TTGCTGCTGCTTGACAGATT-TAMRA-3’ | Probe | ||
| 6 | SARS-CoV-2 SgEF | CGATCTCTTGTAGATCTGTTCTC | 171 | E |
| SARS-CoV-2 SgER | ATATTGCATTGCAGCAGTACGCACA | |||
| SARS-CoV-2 SgEP | 5’-FAM-ACACTAGCCATCCTTACTGCGCTTGC-TAMRA-3’ | Probe | ||