TABLE 2.
Primers and PCR conditions for RT-PCR.
| Target Gene | Sequence (5’—3′) | Ta (°C) | Reference |
|---|---|---|---|
| IL-1β (F) | TCCTCCAGCCAGAAAGTGA | 57.0 | Morris et al. (2014) |
| IL-1β (R) | CAGGCGGTAGAAGATGAAGC | ||
| IFN-γ (F) | GTGAAGAAGGTGAAAGTATCATGGA | 57.0 | Kaiser, Underwood and Davison, (2003) |
| IFN-γ (R) | GCTTTGCGCTGGATTCTCA | ||
| IL-10 (F) | CATGCTGCTGGGCCTGAA | 57.5 | Rothwell et al. (2004) |
| IL-10 (R) | CGTCTCCTTGATCTGCTTGATG | ||
| iNOS (F) | AGTGGTATGCTCTGCCTGCT | 60.0 | Selvaraj and Klasing, (2006) |
| iNOS (R) | CCAGTCCCATTCTTCTTCC | ||
| TGF-β (F) | AGGATCTGCAGTGGAGTGGAT | 54.0 | Han et al. (2020b) |
| TGF-β (R) | CCCCGGGTTGTGTTGGT | ||
| IL-6 (F) | CAAGGTGACGGAGGAGGAC | 57.5 | Hong et al. (2012) |
| IL-6 (R) | TGGCGAGGAGGGATTTCT | ||
| TNF-α (F) | ATCCTCACCCCTACCCTGTC | 56.0 | Han et al. (2020b) |
| TNF-α (R) | GGCGGTCATAGAACAGCACT | ||
| IL-17 (F) | GCAGATGCTGGATGCCTAAC | 55.5 | Markazi et al. (2018) |
| IL-17 (R) | ATGGAGCCAGTGAGCGTTT | ||
| TLR 4 (F) | ACCTACCCATCGGACACTTG | 60.0 | Markazi, et al. (2018) |
| TLR 4 (R) | TGCCTGAGAGAGGTCAGGTT | ||
| TLR 5 (F) | CCTTGTGCTTTGAGGAACGAGA | 52.3 | Xu et al. (2015) |
| TLR 5 (R) | CACCCATCTTTGAGAAACTGCC | ||
| K60 (F) | ATTTCCTCCTGCCTCCTACA | 55.0 | Hong et al. (2006) |
| K60 (R) | GTGACTGGCAAAAATGACTCC | ||
| Claudin-1 (F) | TGTAGCCACAGCAAGAGGTG | 55.0 | Chen et al. (2017) |
| Claudin-1 (R) | GACAGCCATCCGCATCTTCT | ||
| Zona Occludens-1 (F) | TGTAGCCACAGCAAGAGGTG | 55.0 | Oxford and Selvaraj, (2019) |
| Zona Occludens-1 (R) | CTGGAATGGCTCCTTGTGGT | ||
| RPS13 (F) | CAAGAAGGCTGTTGCTGTTCG | 55.5 | Hutsko. (2017) |
| RPS13 (R) | GGCAGAAGCTGTCGATGATT |