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Occurrence of target and non-target sequence alignment for published primer (F′ forward, R′ reverse) and probe (P′) sequences targeting BRD associated bacterial pathogens H. somni; Histophilus somni, P. multocida; Pasteurella multocida, T. pyogenes; Trueperella pyogenes, M. bovis; Mycoplasma bovis, M. haemolytica; Mannheimia haemolytica, U. diversum; Ureaplasma diversum. Fisher’s exact test; p > 0.05 ns, p ≤ 0.05 *, p ≤ 0.01 **, p ≤ 0.001 ***.
