TABLE 3.
Reporting of optimization, analytical and diagnostic sensitivity and specificity, PCR method, quantification and MIQE guideline application (Table 2). Se, sensitivity; Sp, specificity.
| PCR Method Reported | Optimization | Analytical | Diagnostic | Quantification | MIQE Guidelines Applied | References | ||
|---|---|---|---|---|---|---|---|---|
| Se | Sp | Se | Sp | |||||
| qPCR | ✓ | ✓ | ✓ | ✓ | ✓ | ✗ | ✓ | Andres-Lasheras et al. (2020) |
| Multiplex qRT-PCR | ✗ | ✓ | ✓ | ✗ | ✗ | ✗ | ✗ | Goto et al. (2020) |
| Multiplex real-time RT-PCR | ✓ | ✓ | ✓ | ✗ | ✗ | ✗ | ✗ | Horwood and Mahony, (2011) |
| Taqman real-time PCR | ✗ | ✓ | ✓ | ✗ | ✗ | ✗ | ✗ | Kishimoto et al. (2017) |
| NanoPCR | ✓ | ✓ | ✓ | ✗ | ✗ | ✗ | ✗ | Liu et al. (2019) |
| Multiplex real-time PCR | ✓ | ✓ | ✓ | ✗ | ✗ | ✗ | ✓ | Loy et al. (2018) |
| PCR & RT-PCR (both multiplex) | ✓ | ✓ | ✓ | ✗ | ✗ | ✗ | ✗ | Thanthrige-Don et al. (2018) |
| Multiplex RT-PCR | ✓ | ✓ | ✓ | ✗ | ✗ | ✗ | ✓ | Thonur et al. (2012) |
| RT-qPCR | ✓ | ✓ | ✓ | ✗ | ✗ | ✗ | ✗ | Xie et al. (2021) |
| Multiplex PCR | ✓ | ✓ | ✓ | ✗ | ✗ | ✗ | ✗ | Zhang et al. (2017) |
| RT-PCR | ✗ | ✓ | ✓ | ✗ | ✗ | ✗ | ✗ | Socha and Rola, (2011) |
| GeXP-multiplex PCR | ✓ | ✓ | ✓ | ✗ | ✗ | ✗ | ✗ | Fan et al. (2017) |
| Multiplex PCR | ✓ | ✓ | ✓ | ✓ | ✗ | ✗ | Nefedchenko et al. (2016) | |
| PCR | ✗ | ✓ | ✓ | ✗ | ✗ | ✗ | ✗ | Szacawa et al. (2015) |