TABLE 5.

Continued Published PCR primer and probe sequences for detection of viral pathogens associated with bovine respiratory disease complex. F′ forward; P′ probe, R’ reverse sequences.

Pathogen	Target Gene	Sequence (5’—3′)	References
Bovine Rhinitis A Virus	3Dpol	F′ CAC​CTG​AAC​TAT​GGA​CTT​GG	Kishimoto et al. (2017)
		P′ GAC​GTG​GAC​TGG​CAC​CAG​TTT​GC
		R′ CACGGCCTCAATCATCTG
Bovine Rhinitis B Virus	3Dpol	F′ AAC​GCG​ATT​GTG​TCC​TAG​GG	Kishimoto et al. (2017)
		P′ CTG​TCC​TTT​GCA​CGG​CGT​GG
		R′ GCC​ACT​GAG​GTT​AGC​TTC​TC
	3D gene	F′ CGTGGCACACTTCAGGAG	Xie et al. (2021)
		P′ TRGCRGGTCTCGCTTTYCACAGT
		R′ GTGTACCCAYCTCARACGAAG
Bovine Viral Diarrhea Virus	5′UTR	F′ GRAGTCGTCARTGGTTCGAC	Goto et al. (2020)
		P′ TGCYAYGTGGACGAGGGCATGC
		R′ TCA​ACT​CCA​TGT​GCC​ATG​TAC
	5′UTR	F′ TGG​ATG​GCT​TAA​GCC​CTG​AGT​A	Horwood and Mahony, (2011)
		P′ AGTCGTCAGTGGTTCGA
		R′ CCTCGTCCACGTGGCATC
	5′UTR	F′ GGGNAGTCGTCARTGGTTCG	Kishimoto et al. (2017)
		P′ CCAYGTGGACGAGGGCAYGC
		R′ GTGCCATGTACAGCAGAGWTTTT
	5′UTR	F′ CAT​ACC​TTC​AGT​AGG​ACG​AGC	Thanthrige-Don et al. (2018)
		R′ ATG​TGC​CAT​GTA​CAG​CAG​AG
	5′UTR	F′ CATGCCCRYAGTAGGACTAGC	Thanthrige-Don et al. (2018)
		R′ ATG​TGC​CAT​GTA​CAG​CAG​AG
	5′UTR	F′ GTGAGTTCGTTGGATGGC	Fan et al. (2017)
		R′ TAT​GTT​TTG​TAT​AAG​AGT​TCA​TTT​G