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. 2022 Jul 18;13:931562. doi: 10.3389/fgene.2022.931562

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Copyright © 2022 Luo, Luo, Li, Chen, Wu, Chen, Shen, Li, Guo, Yin, Zhang and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Luciferase assay for the putative promoter of ACE2 in HEK293T and A549 cells. Two constructs representing rs2106809 genotype GG and genotype AA harbored in the promoter region (∼1.33kb; chrX: 15 599 868–15 601 196; hg38) were, respectively, inserted into the pGL3-Basic vector and then transfected into HEK293T and A549 cells separately. A Renilla luciferase vector was co-transfected as an internal control. The luciferase activity was measured at 24 h post transfection. The ratio of firefly to Renilla bioluminescence is presented as the mean + SD of triplicated transfection in one representative experiment (*: p < 0.05).