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. 2022 Jul 1;41(15):e110218. doi: 10.15252/embj.2021110218

Figure 1. CRIP1 is upregulated in HCC and associated with poor survival.

Figure 1

  • A
    Western blot analysis of CRIP1 protein expression in 16 paired HCC tumor tissues (T) and adjacent nontumor tissues (N). The graph represents CRIP1 protein expression normalized to GAPDH levels (right).
  • B
    Representative IHC images of CRIP1 protein expression in 108 paired HCC tumor tissues and adjacent nontumor tissues. Scale bars: 20 μm. Statistical analysis of immunohistochemical score of CRIP1 expression in HCC tumor tissues and paired adjacent tissues (right).
  • C
    CRIP1 mRNA expression in the TCGA‐LIHC dataset.
  • D
    mRNA levels of CRIP1 in the GSE36376 dataset from the GEO database.
  • E, F
    The mRNA (E) and protein (F) levels of CRIP1 in HCC cell lines and normal LO2 hepatocytes.
  • G
    Relative CRIP1 protein level compared to normal LO2 hepatocytes.
  • H, I
    Kaplan–Meier survival analyses of OS (H) and PFS (I) based on CRIP1 expression levels in 108 HCC patients.

Data information: Data represent the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001. Differences were tested using a two‐tailed, paired (A, B) and unpaired (C–E, G) Student's t‐test and the log‐rank test (H, I).