Figure 5. Codon‐optimization and ste13 ⁺ deletion do not significantly affect the steady‐state mRNA number of mad1 ⁺ .

• A
  Schematic of the mad1 ⁺ gene. Regions coding for important structural features are highlighted. Black lines in the bottom graph indicate synonymous codon changes in the codon‐optimized version.
• B
  Scatter plots of whole‐cell mRNA counts versus cell length. Solid lines are regression curves from generalized linear mixed model fits (gray: wild type, black: codon‐optimized or ste13Δ). Dashed lines: 95% bootstrap confidence bands for the regression curves. Model estimates of the ratio relative to wild‐type mRNA are included with bootstrap 95% confidence interval in brackets. Two to three replicates per genotype.
• C
  Time course of RNA abundances by qPCR following metabolic labeling and removal of the labeled pool (two independent experiments). Solid lines: regression curves from generalized linear mixed model fits (dark = ste13 ⁺, light = ste13Δ), excluding t = 0 to accommodate for non‐instantaneous labeling by 4tU. Shaded area: 95% bootstrap confidence band for ste13 ⁺; dashed lines: 95% bootstrap confidence band for ste13Δ. Half‐life estimates are included with 95% bootstrap confidence intervals in brackets. See Fig EV4E for additional statistics. The ste13 ⁺ data are the same as in Fig 2.
• D
  Comparison between mean CSC values for selected genes (CSC_g) and mRNA half‐life measured with or without deletion of ste13 ⁺. mRNA half‐life estimates from Figs 3E and 5C, and EV4D.

Source data are available online for this figure.