Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental models
Schizosaccharomyces pombe strains	This study	Appendix Table S1
Saccharomyces cerevisiae strain	Nick Buchler, NC State University, USA	Appendix Table S1
Recombinant DNA
sgRNA sequences	This study	Appendix Table S2
Codon‐optimized mad1, mad2, and mad3	This study	Appendix Table S3
PCR fragments for in vitro transcription	This study	Appendix Table S6
Antibodies
Mouse anti‐Cdc13 (monoclonal)	Novus	Cat # NB200‐576; RRID: AB_10003103
Rabbit anti‐Cdc2 (polyclonal)	Santa‐Cruz	Cat # sc‐53; RRID: AB_2074908
Mouse anti‐GFP (mix of 2 monoclonals)	Roche	Cat # 11814460001; RRID: AB_390913
Rabbit anti‐Mad1 (polyclonal, against peptide ADSPRDPFQSRSQLC)	Heinrich et al (2013), PMID: 24161933	N/A
Rabbit anti‐Mad2 (polyclonal, against recombinant protein)	Sewart and Hauf (2017), PMID: 28366743	N/A
Rabbit anti‐Mad3 (polyclonal, against recombinant protein)	Sewart and Hauf (2017), PMID: 28366743	N/A
Rabbit anti‐Strep‐tag II (monoclonal, recombinant)	Abcam	Cat # ab180957
Rabbit anti‐Strep‐tag II (polyclonal)	Abcam	Cat # ab76949; RRID: AB_1524455
Mouse anti‐tubulin	Sigma	Cat # T5168; RRID: AB_477579
Goat anti‐mouse HRP	Jackson ImmunoResearch Labs	Cat # 115–035‐003; RRID: AB_10015289
Goat anti‐rabbit HRP	Jackson ImmunoResearch Labs	Cat # 111–035‐003; RRID: AB_2313567
Oligonucleotides and other sequence‐based reagents
FISH probes	This study	Appendix Table S4
qPCR primers	This study	Appendix Table S5
Chemicals, enzymes, and other reagents
4‐thiouracil (4tU)	Chem Impex	Cat # 21484
MTSEA biotin‐XX	Biotium	Cat # 90066
Cycloheximide (from Streptomyces griseus)	Chem Impex	Cat # 00083
Wizard SV Gel and PCR Clean‐Up System	Promega	Cat # A9285
SuperScript IV First Strand Synthesis System	ThermoFisher	Cat # 18091050
HiScribe T7 ARCA mRNA Kit (with tailing)	New England Biolabs	Cat # E2060S
Monarch RNA Cleanup Kit	New England Biolabs	Cat # T2040S
Rabbit Reticulocyte Lysate, Nuclease‐Treated	Promega	Cat # L4960
EasyTag EXPRESS 35S Protein Labeling Mix	Perkin Elmer	Cat # NEG772007MC
SUPERase•In RNase Inhibitor	ThermoFisher	Cat # ACM2694
SuperSignal West Pico PLUS Chemiluminescent Substrate	ThermoFisher	Cat # 34580
cOmplete, EDTA‐free Protease Inhibitor Cocktail	Roche	Cat # 04693132001
Halt Protease Inhibitor Cocktail, EDTA‐Free (100X)	ThermoFisher	Cat # 87785
PhosSTOP	Roche	Cat # 04906837001
Halt Phosphatase Inhibitor Cocktail	ThermoFisher	Cat # 78420
Dynabeads Protein G	ThermoFisher	Cat # 10003D
Dynabeads His‐Tag Isolation and Pull‐down	ThermoFisher	Cat # 10103D
MagStrep “type3” XT beads	IBA Lifesciences	Cat # 2–4090‐002
Dynabeads MyOne Streptavidin C1	Thermo Fisher	Cat # 65001
Oligo d(T)25 Magnetic Beads	New England Biolabs	Cat # S1419S
Pierce BCA Protein Assay Kit	ThermoFisher	Cat # 23225
EMM (Edinburgh's Minimal Medium)	MP Biomedicals	Cat # 114110022
Lectin	Sigma	Cat # L1395
Software
Fiji/ImageJ	Schindelin et al (2012), PMID: 22743772	https://imagej.net/software/fiji/; RRID: SCR_002285
SoftWoRx	Applied Precision, GE Healthcare	https://download.cytivalifesciences.com/cellanalysis/download_data/softWoRx/6.5.2/SoftWoRx.htm; RRID: SCR_019157
MetaMorph	Molecular Devices	Version 7.10.1
YeaZ	Dietler et al (2020), PMID: 33184262	N/A
ImageLab	Bio‐Rad Laboratories	Version 6.0.1 build 34
Matlab	Mathworks	https://www.mathworks.com ; RRID: SCR_001622
FISH‐Quant	Mueller et al (2013), PMID: 23538861	N/A
Trainable Weka Segmentation	Arganda‐Carreras et al (2017), PMID: 28369169	N/A
Prism 9	GraphPad Software, Inc	https://www.graphpad.com ; RRID: SCR_002798
R	Cran.R	https://cran.r‐project.org ; RRID: SCR_001905
R studio	N/A	https://www.rstudio.com ; RRID: SCR_000432
tidyverse package	Cran.R	https://tidyverse.tidyverse.org ; RRID: SCR_019186, Version 1.3.1
ggplot2 package	Cran.R	https://ggplot2.tidyverse.org/ ; RRID: SCR_014601
alphashape3d package	Cran.R	https://CRAN.R‐project.org/package=alphashape3d, Version 1.3.1
boxcoxmix package	Cran.R	https://cran.r‐project.org/src/contrib/Archive/boxcoxmix/, Version 0.28
broom package	Cran.R	https://CRAN.R‐project.org/package=broom, Version 0.7.9
broom.mixed package	Cran.R	https://CRAN.R‐project.org/package=broom.mixed, Version 0.2.7
cairo package	Cran.R	https://CRAN.R‐project.org/package=Cairo, Version 1.5–12.2
cowplot package	Cran.R	https://cran.r‐project.org/package=cowplot ; RRID: SCR_018081, Version 1.1.1
descTools package	Cran.R	https://cran.r‐project.org/package=DescTools, Version 0.99.43
egg package	Cran.R	https://CRAN.R‐project.org/package=egg, Version 0.4.5
geometry package	Cran.R	https://CRAN.R‐project.org/package=geometry, Version 0.4.5
gridExtra package	Cran.R	https://CRAN.R‐project.org/package=gridExtra, Version 2.3
lemon package	Cran.R	https://CRAN.R‐project.org/package=lemon, Version 0.4.5
lme4 package	Cran.R	https://cran.r‐project.org/web/packages/lme4/index.html; RRID: SCR_015654
Irescale package	Cran.R	https://CRAN.R‐project.org/package=Irescale, Version 2.3.0
MASS package	Cran.R	https://cran.r‐project.org/package=MASS ; RRID: SCR_019125
mclust package	Cran.R	https://cran.r‐project.org/package=mclust
nabor package	Cran.R	https://cran.r‐project.org/package=nabor
pbkrtest package	Cran.R	https://cran.r‐project.org/package=pbkrtest
plotly package	Cran.R	https://plotly.com/r/; RRID: SCR_013991, Version 4.10.0
plyr package	Cran.R	https://cran.r‐project.org/package=plyr
readxl package	Cran.R	https://cran.r‐project.org/web/packages/readxl/index.html ; RRID: SCR_018083, Version 1.3.1
rgl package	Cran.R	https://CRAN.R‐project.org/package=rgl, Version 0.107.14
sf package	Cran.R	https://CRAN.R‐project.org/package=sf
shotGroups package	Cran.R	https://CRAN.R‐project.org/package=shotGroups, Version 0.8.1
spatstat package	Cran.R	https://cran.r‐project.org/package=spatstat
Other
Mixer mill MM400	Retsch	Cat # 20.745.0001
Grinding jar 10 ml	Retsch	Cat # 01.462.0236
Grinding jar 25 ml	Retsch	Cat # 01.462.0213
Adapter for reaction vials	Retsch	Cat # 22.008.0008
Glass beads, acid‐washed	Sigma	Cat # G8772
μ‐Slide 8‐well, glass bottom	Ibidi	Cat # 80827
Y04C Microfluidic Plate for Haploid Yeast	CellAsic / Sigma	Cat # Y04C‐02‐5PK
Invitrogen NuPAGE 4 to 12%, Bis‐Tris, 20‐well	Invitrogen	Cat # WG1402BOX
Invitrogen NuPAGE 4 to 12%, Bis‐Tris, 20‐well	Invitrogen	Cat # NP0322BOX
Immobilon‐P PVDF membrane	Millipore	Cat # IPVH00010