TABLE 3.
Localization of GFP-FtsI rings in fts temperature-sensitive mutants
| Mutanta | Growth temperature (duration) | No. of cells scored | Cell length (avg ± SD) (μm) | Total no. of FtsI rings | % of cells with ring(s) | Spacing of FtsI ringsc (μm of cell length/ring) |
|---|---|---|---|---|---|---|
| Wild type | 30°C | 381 | 4.1 ± 1.0 | 221 | 58 | 7.1 |
| 42°C (60 min) | 338 | 4.5 ± 1.4 | 146 | 43 | 10 | |
| ftsZ(Ts) | 30°C | 265 | 5.4 ± 1.8 | 112 | 42 | 13 |
| 42°C (60 min) | 127 | 23.9 ± 6.2 | 4b | 3 | 760 | |
| ftsA(Ts) | 30°C | 224 | 4.9 ± 1.2 | 128 | 57 | 8.6 |
| 42°C (60 min) | 96 | 24.4 ± 5.1 | 2b | 2 | 1,200 | |
| ftsQ(Ts) | 30°C | 116 | 9.6 ± 5.1 | 20b | 17 | 56 |
| 42°C (45 min) | 110 | 22.8 ± 7.8 | 5b | 5 | 500 |
a
Strains used were EC454 for the wild type, EC458 for ftsZ84(Ts), EC455 for ftsA12(Ts), and EC457 for ftsQ1(Ts).
b
Faint.
c
Total length of cells (or filaments) divided by total number of FtsI rings.