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. 2022 Jul 11;149(13):dev200184. doi: 10.1242/dev.200184

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Shared gene expression features of gills and the pseudobranch in skate. (A) In skate, interbranchial septa bearing gill lamellae are internally supported by cartilaginous branchial rays. Frontal section stained with modified Masson's trichrome. (B-Biii) Putative hypoxia-sensitive neuroepithelial cells (NECs) co-expressing serotonin and SV2 are present in S32 skate gill lamellae. Immunofluorescence on a frontal section. (C-E) Skate gills are marked by the expression of hif2a (C), gata3 (D) and gcm2 (E), as detected by mRNA in situ hybridisation by chain reaction on frontal sections. (F) The skate pseudobranch is internally supported by the spiracular cartilage. Frontal section stained with modified Masson's trichrome. (G-Giii) NECs co-expressing serotonin and SV2 are also present in the lamellae of the skate pseudobranch. Immunofluorescence on a frontal section. (H-J) The skate pseudobranch shares expression of hif2a (H), gata3 (I) and gcm2 (J) with gills, as detected by mRNA in situ hybridisation by chain reaction on frontal sections. br, branchial rays; sc, spiracular cartilage; pb, pseudobranch. Scale bars: 50 μm in A,B,C-E,F,G,H-J; 5 μm in Bi-Biii, Gi-Giii.