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. 2022 Jul 11;149(13):dev200184. doi: 10.1242/dev.200184

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — The skate spiracular cartilage derives from GAER shh-responsive mandibular arch mesenchyme. (A-D) Microinjection of the lipophilic dye CM-DiI in the mandibular arch mesenchyme subjacent to the shh-expressing GAER (B) results in focal labelling of ptc+ (i.e. shh responsive) mesenchyme of the posterior mandibular arch (C,D). (E-F) At 60 days post-injection, CM-DiI-positive chondrocytes are recovered throughout the spiracular cartilage (F). ma, mandibular arch. Scale bars: 50 μm in A-E,F; 5 μm in Ei,Eii.