TABLE 2.

Localization frequencies of and complementation by GFP-FtsQ and GFP-FtsQ swap protein constructs

Construct	Structurea	Total no. of cells scored	% Localization frequency (mean ± SD)b	Complementation of the following mutation:
				Tsc	Nulld
GFP-FtsQ		715	60 ± 3	+	+
GFP-QQQ		696	44 ± 7	+	+
GFP-FQQ		703	37 ± 8	+	+
GFP-FFQ		654	35 ± 10	+	±
GFP-QFQ		599	30 ± 6	+	±
GFP-QQL		150	0	−	ND
GFP alone		60	0	−	−

^aOpen boxes represent domains of FtsQ. Hatched boxes represent domains derived from MalF. Stippled boxes denote GFP. The box with vertical bars represents the periplasmic domain of FtsL. E and M indicate the borders of the transmembrane domain where the restriction sites for EagI and MscI, respectively, were introduced. These sites caused the following amino acid changes in FtsQ: T23G, W48L, M49G, and E50D. Diagrams of fusion proteins are not to scale. The cytoplasmic and transmembrane domains of FtsQ contained 24 and 25 amino acids, respectively, while those of MalF contained 16 and 25 amino acids. The periplasmic domain of FtsQ contained 227 amino acids, while that of FtsL contained 64 amino acids. 

^bDetermined from three independent sets of experiments. Strains EC442, JOE192, JOE193, JOE194, JOE196, and JOE257 were grown in parallel cultures, fixed, and scored for localization as described in Materials and Methods. 

^cComplementation of the ftsQ1(Ts) allele was determined as described in Materials and Methods. The strains used were JOE225, JOE226, JOE228, JOE229, JOE230, JOE231, and JOE259. +, complementation; −, no complementation. 

^dComplementation of the ftsQ::TnphoA50 null allele was determined as described in Materials and Methods. The strains used were JOE204, JOE206, JOE210, JOE212, JOE214, and JOE216. +, complementation; ±, partial complementation; −, no complementation; ND, not determined.