Skip to main content
. 2022 Jul 13;65(14):9718–9734. doi: 10.1021/acs.jmedchem.2c00142

Figure 4.

Figure 4

Effect of compound 1 and analogues on AGTwt and the G41R variant expressed in mammalian cells. CHO-GO-AGTwt and CHO-GO-AGT-G41R cells were grown for 7 days in the presence of 50 μM of compound 1 or its analogues. At the end of treatment, cells were detached and lysed, and soluble fraction of each sample was used for (A) transaminase activity determination. The values in CHO-GO-AGTwt control cells (191 ± 10 nmol of pyruvate/min/mg protein) and in CHO-GO-AGT-G41R control cells (6.8 ± 0.7 nmol of pyruvate/min/mg protein) were assumed to be 1 to facilitate the assessment of the changes. Data represent mean ± SEM (n = 3). *p < 0.05 vs control cells. (B) AGT protein level quantification by western blot. AGT levels in CHO-GO-AGTwt and CHO-GO-AGT-G41R control cells were assumed to be 1 to facilitate the assessment of the changes. GAPDH was used as loading control. The images are representative of one out of three separate experiments. Data represent mean ± SEM (n = 3). *p < 0.05 vs control cells.