TABLE 1.

Primer sequences used in the construction of deletions and mutations

Primer	Sequence (5′ to 3′)a	Locationb
P1	CCTTTTAAGGATCCGCCGTCTGAAAATTCACAAAATATGAATGTTA	−364
P2	GGCTAATGGATCCCGTTTCATAATGTTTTCCTTTTG	+31
P3	GATGAAACGCCGAGTTAACGCC	+470c
P4	ACTTAAGGATCCATGCCCGCAATGGGACAACC	−15
P5	ATGAATGGATCCGATTGTAGCATGGTTTACCG	−250
P6	ACCGCAATTACACACCAAGTTCTTAACTAATC	−7
P7	TGTGTAATTGCGGTTGTCCCATTGCGGGC	+15
P8	TCACACGTTCATCTTAACTAATCC	+15
P9	AAGATGAACGTGTGATTATGCGGTTG	−19
P10	CAGGTTATTTGACGTAAATTAAAATGC	−31
P11	CGTCAAATAACCTGTAAAATATGAAC	−69
P12	AATCTCTAGACACCAAGTTCTTAACTAATC	+14
P13	TGGTGTCTAGAGATTATGCGGTTGTCCCATTG	−27
P14	AAAGGATCAAATAATGAAACGGGATCCCGTCG	+65C
P15	GATCCTTTGTATAGAAAAGTAGGGGGGATTAG	+9
P16	CTTAAAATTTTATGCCCGCAATGGGAC	−19
P17	GGGCATAAAATTTTAAGTCAAATAATTC	−46
P18	CCGGAGGGATCCGCAAATCAGCCTATTCATTG	−111
P19	ATGAATTATCTAACTTAAATTAAAATGC	−31
P20	TAAGTTAGATAATTCATAAAATATGAAC	−69
P21	TTTTATGGATCCTTTGACTTAAATTAAAATGC	−31
P22	GTCATTTTGGATCCATATTTTATGAATTATTTGAC	−45
P23	ATCGTACTCGAGCCAGTAGTTCGGGCGGCCTTT	+2650
P24	TAATGTCTCGAGTTTGTAAGAAAAGTAGGGGGGAT	+12
P25	ACAAAAGGATCCCATTATGAAACGCCAAGCAT	+62
P26	TTATAAGGATCCAGAAGCGTCATTTTAAGTTC	−65

^aChanges in the sequence from the wild-type sequence are indicated by bold type. 

^bLocation of the 3′ end of the primer relative to the sigma 70-type promoter transcription start site. 

^cRelative to the sigma 70-type promoter transcription start site in the lacZ fusion.