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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Cell Rep. 2020 May 12;31(6):107632. doi: 10.1016/j.celrep.2020.107632

Figure 4. Oxidative-Stress-Induced DDR and Proliferation in ISCs/EBs Required OGT.

Figure 4.

(A) After 6 days of induction at 29C, immunofluorescence staining of esg-GFP (green) in midguts of esgts>+ and esgts>OGTRNAi transgene flies followed by treatment with 10 mM PQ in 5% sucrose or 5% sucrose alone for 20 h at 29°C.

(B) The number of PH3-positive cells in midguts from flies.

(C) Immunofluorescence staining images of O-GlcNAc (red) in esg-GFP-positive cells (green) in midgut of flies from the indicated genotype and treatment.

(D) Quantification of O-GlcNAc mean fluorescence per esg-GFP-positive cell.

(E) Immunofluorescence staining of γH2AvD (red) in midgut of flies from the indicated genotype and treatment.

(F) Quantification of γH2AvD mean fluorescence per esg-GFP-positive cell.

(G) Immunofluorescence staining of pS/Tq (red) in midgut of flies from the indicated genotype and treatment.

(H) Quantification of pS/Tq mean fluorescence per esg-GFP-positive cell.

Outline indicates esg-positive cell boundary. Data are represented as mean ± SD. ****p < 0.0001. n.s., not significant; see Table S1 for N values.