REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-coronavirus (SARS-CoV-2) spike S2 mouse mAb	MP Biomedicals	Cat#08720401; RRID: AB_2920626
PE-labeled anti-his antibody	Miltenyi	Cat#130120787; RRID: AB_2752196
FITC-labeled anti-mouse IgG	ThermoFisher Scientific	Cat# A16073; RRID: AB_2534746
Chemicals, peptides, and recombinant proteins
Fetal bovine serum	GIBCO	Cat#16000-044
Trypsin	Macgene	Cat#CC017
ACE2 recombinant protein	This paper	N/A
Recombinant coronavirus RBDs	This paper	N/A
Cellfectin II Reagents	GIBCO	Cat#10362100
High Affinity Ni-Charged Resin FF	Genscript	Cat# L00666
Critical commercial assays
Bright-Glo™ Luciferase Assay Buffer	Promega	Cat#E264B
Bright-Glo™ Luciferase Assay Substrate	Promega	Cat#E263B
Deposited data
Horse ACE2 with SARS-CoV-2 RBD	This paper	PDB: 7FC5
Horse ACE2 with SARS-CoV RBD	This paper	PDB: 7FC6
Horse ACE2 with hCoV-NL63 RBD	This paper	PDB: 7FC3
Experimental models: Cell lines
Human: HEK293T, embryo	ATCC	CRL-3216
Human: HeLa, Female	ATCC	CCL-2
HeLa expressing ACE2 from diverse origin, Female	Dr. Qiang Ding’s lab	Liu et al., 2021c
Spodoptera frugiperda cells	ATCC	CRL-1711
Trichoplusia ni cells	ATCC	PTA-9384
Oligonucleotides
eACE2for: TCCCACCATCGGGCGCGGATC CATGAGCGGCAGCTCCTGGCTGCT	RuiBiotech	N/A
eACE2rev: CCTCTAGTACTTCTCGACAAGC TTTCAATGGTGATGGTGATGGTGGTCGGC GTAGGGGGACC	RuiBiotech	N/A
eACE2-H41Yfor: CTGAGCTACCAGTCTA GCCTGGCCTCT	RuiBiotech	N/A
eACE2-H41Yrev: AGACTGGTAGCTCAGC TCCTCGGCCTCA	RuiBiotech	N/A
SARS-CoV-2for: TCTGCCTTTGCGGCGGATCCCACCAAT CTGTGCCCTTTCGGCGA	RuiBiotech	N/A
SARS-CoV-2rev: TCCTCTAGTACTTCTC GACAAGCTTTCAATGGTGATGGTGATG GTGAGGGCCGCACACGGTA	RuiBiotech	N/A
hCoV-NL63for: TCTGCCTTTGCGGCGG ATCCCCAGCACACCGACATCAACT	RuiBiotech	N/A
hCoV-NL63rev: TCCTCTAGTACTTCTCGACAAGCTTTCAA TGGTGATGGTGATGGTGGATACCGGA CACAGGGT	RuiBiotech	N/A
SARS-CoVfor: TCTGCCTTTGCGGCGGATCCCCGGGT GGTGCCCAGCGGCGAC	RuiBiotech	N/A
SARS-CoVrev: TCCTCTAGTACTTCTCGACAAGCTTTCA ATGGTGATGGTGATGGTGCAGCTTGGG GCCGCACAC	RuiBiotech	N/A
Recombinant DNA
pcDNA3.1	Thermo Fisher	Cat#V79020
pFastBac-Dual vector	GIBCO	Cat#10712024
pLVX-IRES-zsGreen1 vector	Clontech Laboratories, Inc	Cat#632187
pNL4-3-R-E-luciferase	NIH-AIDS Reagent Program	Cat#3418
Software and algorithms
FlowJo 10 software	FlowJo	https://www.flowjo.com/
Graphpad Prism 7	GraphPad	www.graphpad.com
Biacore Evaluation Software	GE Healthcare	https://www.cytivalifesciences.com/en/us/shop/protein-analysis/spr-label-freeanalysis/software/biacore-insightevaluation-software-p-23528
HKL2000	Otwinowski and Minor (1997)	https://www.hkl-xray.com/hkl-2000
PHASER (CCP4 Program Suite)	McCoy et al. (2007)	http://www.phaser.cimr.cam.ac.uk/inind.php/Phaser_Crystallographic_Software
COOT	Emsley and Cowtan (2004)	http://www2.mrc-lmb.cam.ac.uk/Personal/pemsley/coot/
PHENIX	Adams et al. (2002)	http://www.phenix-online.org/
UCSF Chimera X	Pettersen et al. (2021)	https://www.cgl.ucsf.edu/chimerax/
Other
Superdex 200 High-Performance column	GE Healthcare	N/A
Nanodrop One Spectrophotometer	Thermo Scientific	N/A
CM5 sensor chip	GE Healthcare	Cat#BR100530
Biacore T200	GE Healthcare	N/A