Table 6.

Primer sequence, band length, total amplified bands, monomorphic, polymorphic, unique bands and percentage of polymorphism and efficiency of ISSR and SRAP analysis of three A. maurorum genotypes

Primer Name	Primer sequence (5`----3`)	Band length (bp)	TAB	NMB	NPB	NUB	PPB	MBF	PIC	RP
ISSR markers
49A	CAC ACA CAC ACA AG	215–385	2	1	1	–	50%	0.83	0.22	0.67
HB-9	CAC CAC CAC GC	380–435	2	2	–	–	–	1.00	0.00	0.00
HB-10	GAG AGA GAG AGA CC	280–470	2	1	1	–	50%	0.83	0.22	0.67
HB-11	GTG TGT GTG TGT TGT CC	460–810	2	1	1	–	50%	0.83	0.22	0.67
HB-13	GAG GAG GAG C	470–975	3	2	1	–	33.33%	0.89	0.15	0.67
Total		–	11	7	4	–	–	–	–	–
Average		200–900	2.2	1.4	0.8	–	36.36%	0.88	–	0.53
DI of all ISSR primers = 0.16
SRAP markers
ME1xEM7	F ME-1: GAGTCCAAACCGGATA R EM-7: GACTGCGTACGAATTCAA	275–1500	6	–	6	3	100%	0.50	0.45	4.02
ME1xEM8	ME-1: GAGTCCAAACCGGATA EM-8: CTGCGTACGAATTCAC	320–680	4	2	2	–	50%	0.83	0.22	1.36
ME2xEM6	ME-2: TGA GTC CAA ACC GGA GC EM-6: GAC TGC GTA CGA ATT CC	145–845	7	2	5	2	71.42%	0.66	0.32	3.36
ME2xEM7	ME-2: TGA GTC CAA ACC GGA GC Em-7: GACTGCGTACGAATTCAA	135–185	3	2	1	1	33.33%	0.78	0.15	0.66
ME5xEM6	Me-5: TGAGTCCAAACCGGAAG EM-6: GAC TGC GTA CGA ATT CC	110–200	3	1	2	1	66.66%	0.66	0.30	1.34
ME5xEM8	Me-5: TGAGTCCAAACCGGAAG EM-8: CTGCGTACGAATTCAC	100–250	2	2	–	–	–	1.00	0	0
Total		–	25	9	16	7	–	–	–	–
Average		100–1500	4.17	1.5	2.67	1.17	64%	0.74	–	1.79
DI of all SRAP primers = 0.24
Combined ISSR and SRAP			36	16	20	7	55.55%	–	–	–

TAB Total amplified bands, NMB Number of monomorphic bands, NPB Number of polymorphic bands; NUB Number of unique bands, PPB Percentage of polymorphic bands, MBF Mean of band frequency, PIC polymorphism information content, Rp Resolving power and DI Diversity index