Figure 3.

MALDI-IMS ion images of B. subtilis on TSA, BHI, and LB prepared using the three step sprayer method. DHB was used as the ionization matrix and data was acquired in a single acquisition. Putative annotations: m/z 162, 169, 176, 180, 257, and 674 are unknown metabolites; m/z 1074 is a surfactin; m/z 1543 is a plipastatin. All images were normalized to the total ion count and intensities were adjusted to improve visualization between samples. Annotation of compounds from the surfactin and plipastatin molecular families are from ref.¹²