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. 2022 Jul 28;10(7):e005054. doi: 10.1136/jitc-2022-005054

Figure 4.

Figure 4

P329G-targeting CAR T cells combined with a HER2 binder mediated sufficient in vitro effector functions. (A) Schematic representation of P329G-targeting CAR (P329G-CAR), HER2-targeting CAR (HER2-CAR), and CD33-targeting CAR (CD33-CAR). (B) Density of HER2 surface expression on MCF-7 HER2 OE and HCC1569. (C) Proliferative capacity after 72 hours of co-culture normalized to CAR T cells per beads measured by FACS after 24 hours co-culture in the presence of HCC1569 or MCF-7 HER2 OE of three independent donors (n=3). (D) MCF-7 HER2 OE tumor cell killing by different T cell conditions plated at E:T ratio of 2:1 measured over time through xCELLigence. (E) IFN-γ and IL-2 ELISA after 24 hours, 48 hours, and 72 hours of co-culture with MCF-7 HER2 OE for three independent donors (n=3). (F) HCC1569 tumor cell killing by different T cell conditions plated at a E:T ratio of 2:1 measured over time through xCELLigence. (G) IFN-γ and IL-2 ELISA after 24 hours, 48 hours, and 72 hours co-culture with HCC1569 supernatants for three independent donors (n=3). Each experiment was performed in triplicates. Subfigures (D, F) show data of one representative donor out of three independent experiments. Values in all graphs represent means±SEM (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). P values for xCELLigence data in (D, F) are shown for last time point. Only selected p values are shown.