TABLE 1.
Bacterial strains, plasmids, and phages used in this study
| Strain,a plasmid or bacteriophage | Relevant genotype | Construction, source, or reference |
|---|---|---|
| Bacterial strains | ||
| DH5αb | F−endA1 hsdR17 (rK− mK+) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169(φ80dlacΔ(lacZ)M15) | Gibco BRL |
| DDS90 | lon+ rcsA+ rcsB+ rcsA90::lacZ | D. Sledjeski; 19, 24a |
| JT2029 | proCYA221 zaj-403::ΔTn10 rcsA90::lacZ | DDS90 + P1 (SG1030) |
| JT2046 | Δlon-510 rcsA+ rcsB+ rcsA90::lacZ | JT2029 + P1 (SG4144) |
| JT2055 | Δlon-510 rcsA72::ΔTn10 rcsB+ rcsA90::lacZ | JT2046 + P1 (SG23001) |
| JT2056 | Δlon-510 rcsA+ rcsB62::ΔKan rcsA90::lacZ | JT2046 + P1 (SG23002) |
| JT2059 | Δlon-510 rcsA72::ΔTn10 rcsB62::ΔKan rcsA90::lacZ | JT2056 + P1 (SG20806) |
| JT4000 | Δlon-510 | SG1030 + P1 (SG4144) |
| SG1030 | F− Δlac araD proCYA221 zaj-403::ΔTn10 | 33 |
| SG4144c | N99 Δlon-510 | 16 |
| SG20250 | lon+ Δlac | 11 |
| SG20250-λi434 | This study | |
| SG20780 | Δlon-510 cpsB10::lacZ | 3 |
| SG20781 | lon+ cpsB10::lacZ | 3 |
| SG20806 | rcsA72::ΔTn10 | 3 |
| SG21081 | lon+ cps+ rcsA72::ΔTn | 3 |
| SG23002 | rcsB62::ΔKan | 29 |
| WE10 | hns::ΔKan rcsA90::lacZ | DDS90 + P1 (DDS1398) |
| WE12 | Δlon-510 hns::ΔKan rcsA90::lacZ | JT2046 + P1 (DDS1398) |
| WE20 | hns::ΔTn10 | This study |
| WE28 | lon+ rcsA+ rcsB+ hns::ΔTn10 rcsA90::lacZ | DDS90 + P1 (WE20) |
| WE29 | Δlon-510 rcsA+ rcsB+ hns::ΔTn10 rcsA90::lacZ | JT2046 + P1 (WE20) |
| WE30 | lon+ rcsA+ rcsB62::ΔKan hns::ΔTn10 rcsA90::lacZ | JT2058 + P1 (WE20) |
| WE31 | Δlon-510 rcsA+ rcsB62::ΔKan hns::ΔTn10 rcsA90::lacZ | JT2056 + P1 (WE20) |
| WE1002 | lon+ rcsA+ rcsA109::lacZ | SG20250 + λRS45-rcsA109::lacZ |
| WE1003 | lon+ rcsA+ cps103::lacZ | SG20250 + λRS45-cps103::lacZ |
| WE1004 | lon+ rcsA+ cps41::lacZ | SG20250 + λRS45-cps41::lacZ |
| WE1012 | lon+ proCYA221 zaj-403::ΔTn10 rcsA109::lacZ | WE1002 + P1 (SG1030) |
| WE1013 | lon+ proCYA221 zaj-403::ΔTn10 cps103::lacZ | WE1003 + P1 (SG1030) |
| WE1014 | lon+ proCYA221 zaj-403::ΔTn10 cps41::lacZ | WE1004 + P1 (SG1030) |
| WE1022 | Δlon-510 rcsA+ rcsA109::lacZ | WE1012 + P1 (SG4144) |
| WE1023 | Δlon-510 rcsA+ cps103::lacZ | WE1013 + P1 (SG4144) |
| WE1024 | Δlon-510 rcsA+ cps41::lacZ | WE1014 + P1 (SG4144) |
| WE1052 | Δlon-510 rcsA72::ΔTn10 rcsA109::lacZ | WE1022 + P1 (SG21081) |
| WE1053 | Δlon-510 rcsA72::ΔTn10 cps103::lacZ | WE1023 + P1 (SG21081) |
| WE1054 | Δlon-510 rcsA72::ΔTn10 cps41::lacZ | WE1024 + P1 (SG21081) |
| WE1062 | Δlon-510 rcsB62::ΔKan rcsA109::lacZ | WE1022 + P1 (SG23002) |
| WE1063 | Δlon-510 rcsB62::ΔKan cps103::lacZ | WE1023 + P1 (SG23002) |
| WE1064 | Δlon-510 rcsB62::ΔKan cps41::lacZ | WE1024 + P1 (SG23002) |
| WE1082 | Δlon-510 rcsA72::ΔTn10 rcsB62::ΔKan rcsA109::lacZ | WE1052 + P1 (SG23002) |
| WE1083 | Δlon-510 rcsA72::ΔTn10 rcsB62::ΔKan cps103::lacZ | WE1053 + P1 (SG23002) |
| WE1084 | Δlon-510 rcsA72::ΔTn10 rcsB62::ΔKan cps41::lacZ | WE1054 + P1 (SG23002) |
| Plasmids | ||
| pATC400 | pBR322-rcsA+ | 31 |
| pRS415 | bla-T14EcoRI-SmaI-BamHI lacZ | 24 |
| pRS415-cpsBox | pRS415 plus 194-bp cps promoter fragment | This study |
| pRS415-cpsNoBox | pRS415 plus 132-bp cps promoter fragment | This study |
| pRS415-rcsANoBox | pRS415 plus 121-bp rcsA promoter fragment | This study |
| pACYC184-lon::ΔKan | pACYC184-lon plus 1.5-kb Kanr cassette from pUC-4K | This study |
| Bacteriophages | ||
| λRS45 | imm21 ind+ bla′-lacZSC | 24 |
| λcI−imm434 | cI−imm434 | N. Trun |
| λ imm434 | imm434 | N. Trun |
a
All strains are derived from MC4100 (ΔlacU169 araD flbB rel) unless otherwise noted.
b
Commercial strain.
c
National Institutes of Health strain.